PRODUCT CODE: ET1612-39

Recombinant Smad1 Monoclonal Antibody (ET1612-39)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Smad1 on different lysates using anti-Smad1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: Human skeletal muscle <br />
 Lane 2: Hela
  • Western blot analysis of Smad1 on different lysates using anti-Smad1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: Human skeletal muscle <br />
 Lane 2: Hela
  • ICC staining Smad1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Smad1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Smad1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Smad1 antibody. Counter stained with hematoxylin.
Western blot analysis of Smad1 on different lysates using anti-Smad1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Human skeletal muscle
Lane 2: Hela

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Smad1 Monoclonal Antibody (ET1612-39)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, MCF-7, Hela, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD2090

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Mothers against decapentaplegic homolog 1

GENE NAME

SMAD1

SYNONYMS

MAD homolog 1, Mothers against DPP homolog 1, SMAD 1, Smad1, hSMAD1, BSP-1, SMAD1

SEQUENCE SIMILARITIES

Belongs to the dwarfin/SMAD family.

TISSUE SPECIFICITY

Ubiquitous. Highest expression seen in the heart and skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of the C-terminal SVS motif by BMP type 1 receptor kinase activates SMAD1 by promoting dissociation from the receptor and trimerization with SMAD4.; Ubiquitinated by SMAD-specific E3 ubiquitin ligase SMURF1, leading to its degradation. Monoubiquitinated, leading to prevent DNA-binding. Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes. Dephosphorylation, probably by PPM1A, induces its export from the nucleus to the cytoplasm (By similarity).

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. Exported from the nucleus to the cytoplasm when dephosphorylated (By similarity).

FUNCTION

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.