PRODUCT CODE: ET1701-6

Recombinant SIRT5 Monoclonal Antibody (ET1701-6)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

Western blot analysis of SIRT5 on Jurkat cells lysates using anti-SIRT5 antibody at 1/1,000 dilution.
  • Western blot analysis of SIRT5 on Jurkat cells lysates using anti-SIRT5 antibody at 1/1,000 dilution.
Western blot analysis of SIRT5 on Jurkat cells lysates using anti-SIRT5 antibody at 1/1,000 dilution.

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SIRT5 Monoclonal Antibody (ET1701-6)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ084-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

37/22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

NAD-dependent protein deacylase sirtuin-5, mitochondrial

GENE NAME

SIRT5

SYNONYMS

SIRT5

SEQUENCE SIMILARITIES

Belongs to the sirtuin family. Class III subfamily.

TISSUE SPECIFICITY

Widely expressed.

SUBCELLULAR LOCATION

Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm, cytosol. Nucleus. Note=Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus.; [Isoform 1]: Cytoplasm. Mitochondrion.; [Isoform 2]: Mitochondrion.

FUNCTION

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.