PRODUCT CODE: ET1611-80

Recombinant SHP2 Monoclonal Antibody (ET1611-80)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of SHP2 on different lysates using anti-SHP2 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: 293
  • Western blot analysis of SHP2 on different lysates using anti-SHP2 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: 293
  • ICC staining SHP2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SHP2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SHP2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SHP2 antibody. Counter stained with hematoxylin.
Western blot analysis of SHP2 on different lysates using anti-SHP2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SHP2 Monoclonal Antibody (ET1611-80)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, MCF-7, Hela, 293, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN07-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Tyrosine-protein phosphatase non-receptor type 11

GENE NAME

PTPN11

SYNONYMS

PTP-1D, PTP-2C, SHP-2, Shp2, PTPN11, PTP2C, SHPTP2

SEQUENCE SIMILARITIES

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.

TISSUE SPECIFICITY

Widely expressed, with highest levels in heart, brain, and skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity. Dephosphorylates CDC73. Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity).