PRODUCT CODE: ET7107-03

Recombinant SF3B1 Monoclonal Antibody (ET7107-03)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SF3B1 on different lysates using anti-SF3B1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse thymus <br />
 Lane 2: Hela<br />
  • Western blot analysis of SF3B1 on different lysates using anti-SF3B1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: Mouse thymus <br />
 Lane 2: Hela<br />
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-SF3B1 antibody. Counter stained with hematoxylin.
Western blot analysis of SF3B1 on different lysates using anti-SF3B1 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse thymus
Lane 2: Hela

Applications

  • WB

  • IHC-P

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SF3B1 Monoclonal Antibody (ET7107-03)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse thymus tissue lysate, Hela, human colon cancer tissue, human skin tissue, human kidney tissue, mouse small intestine tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB40-32

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

146 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • ICC

  • 1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

Splicing factor 3B subunit 1

GENE NAME

SF3B1

SYNONYMS

SF3b155, SAP 155, SF3B1, SAP155

SEQUENCE SIMILARITIES

Belongs to the SF3B1 family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.; Citrullinated by PADI4.

SUBCELLULAR LOCATION

Nucleus. Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

FUNCTION

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity). May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.