PRODUCT CODE: ET7107-18

Recombinant SET Monoclonal Antibody (ET7107-18)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of SET on K562 cell lysates using anti-SET at 1/500 dilution.
  • Western blot analysis of SET on K562 cell lysates using anti-SET at 1/500 dilution.
  • ICC staining SET in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SET in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SET in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SET antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SET antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-SET antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-SET antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SET antibody. Counter stained with hematoxylin.
Western blot analysis of SET on K562 cell lysates using anti-SET at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SET Monoclonal Antibody (ET7107-18)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, Hela, LOVO, MCF-7, human tonsil tissue, human kidney tissue, human lung cancer tissue, mouse fallopian tube tissue, rat kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB60-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

Protein SET

GENE NAME

SET

SYNONYMS

IGAAD, I-2PP2A, TAF-I, SET

SEQUENCE SIMILARITIES

Belongs to the nucleosome assembly protein (NAP) family.

TISSUE SPECIFICITY

Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.

POST-TRANSLATIONAL MODIFICATION

Isoform 2 is phosphorylated on Ser-15 and Ser-24.; Isoform 2 is acetylated on Lys-11.; Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).; N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated (By similarity).; [Isoform 2]: Cleaved after Lys-176 by GZMA. The cleavage inhibits its nucleosome assembly activity and disrupts the inhibition on NME1.

SUBCELLULAR LOCATION

Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus, nucleoplasm. Note=In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. The SET complex is associated with the endoplasmic reticulum. Following CTL attack and cleavage by GZMA, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium.

FUNCTION

Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.