PRODUCT CODE: ET1706-30

Recombinant SDHB Monoclonal Antibody (ET1706-30)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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+
Western blot analysis of SDHB on different lysates using anti-SDHB antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Human liver<br />
  Lane 2: Rat spleen<br />
  Lane 3: Rat liver<br />
  Lane 4: Mouse spleen<br />
  Lane 5: HepG2
  • Western blot analysis of SDHB on different lysates using anti-SDHB antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Human liver<br />
  Lane 2: Rat spleen<br />
  Lane 3: Rat liver<br />
  Lane 4: Mouse spleen<br />
  Lane 5: HepG2
  • Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-SDHB antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with SDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor&reg; 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of SDHB on different lysates using anti-SDHB antibody at 1/500 dilution.
Positive control:
Lane 1: Human liver
Lane 2: Rat spleen
Lane 3: Rat liver
Lane 4: Mouse spleen
Lane 5: HepG2

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SDHB Monoclonal Antibody (ET1706-30)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human liver tissue, rat spleen tissue lysate, rat liver tissue lysate, mouse spleen tissue lysate, HepG2, rat esophagus tissue, human colon cancer tissue, human kidney tissue, mouse heart tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU02-33

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

SDHB

SYNONYMS

CWS2 antibody; DHSB_HUMAN antibody; FLJ92337 antibody Ip antibody; Iron sulfur subunit antibody; Iron sulfur subunit of complex II antibody; Iron-sulfur subunit of complex II antibody; mitochondrial antibody; PGL 4 antibody; PGL4 antibody; SDH 1 antibody; SDH antibody; SDH1 antibody; SDH2 antibody; SDH2, homolog of antibody; SdhB antibody; SDHIP antibody; Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody; Succinate dehydrogenase [ubiquinone] iron sulfur subunit antibody; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody; succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody; Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody; Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody; Succinate dehydrogenase complex iron sulfur subunit B antibody; Succinate dehydrogenase complex subunit B iron sulfur antibody; Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody; succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody; Succinate dehydrogenase iron sulfur protein antibody

SEQUENCE SIMILARITIES

Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.

SUBCELLULAR LOCATION

Mitochondrion. Mitochondrion inner membrane.

FUNCTION

In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations associate with juvenile encephalopathy as well as Leigh syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.