PRODUCT CODE: ET1703-40

Recombinant SDHA Monoclonal Antibody (ET1703-40)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of SDHA on Jurkat cells lysates using anti-SDHA antibody at 1/500 dilution.
  • Western blot analysis of SDHA on Jurkat cells lysates using anti-SDHA antibody at 1/500 dilution.
  • Western blot analysis of SDHA on different cell lysates using anti-SDHA antibody at 1/500 dilution.Positive control: <br />
 Lane 1:HepG2<br />
 Lane 2:SHG44
  • ICC staining SDHA in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SDHA in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SDHA in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-SDHA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-SDHA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SDHA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-SDHA antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with SDHA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of SDHA on Jurkat cells lysates using anti-SDHA antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SDHA Monoclonal Antibody (ET1703-40)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, MCF-7, HepG2, NIH-3T3, Hela, human liver tissue, human kidney tissue, mouse testis tissue, mouse skeletal muscle tissue, mouse colon tissue, zebrafish tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-83

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

68 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SDHA

SYNONYMS

CMD1GG antibody; DHSA_HUMAN antibody; Flavoprotein subunit of complex II antibody; Fp antibody; PGL5 antibody; SDH 2 antibody; SDH1 antibody; SDH2 antibody; SDHA antibody; SDHF antibody; Succinate dehydrogenase [ubiquinone] flavoprotein subunit antibody; Succinate dehydrogenase [ubiquinone] flavoprotein subunit mitochondrial antibody; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial antibody; Succinate dehydrogenase complex flavoprotein subunit A antibody; Succinate dehydrogenase complex flavoprotein subunit antibody; Succinate dehydrogenase complex flavoprotein subunit precursor antibody; Succinate dehydrogenase complex subunit A antibody; Succinate dehydrogenase complex subunit A flavoprotein (Fp) antibody; Succinate dehydrogenase complex subunit A flavoprotein antibody

SEQUENCE SIMILARITIES

Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation.; Acetylated. Deacetylated by SIRT3.

SUBCELLULAR LOCATION

Mitochondrion inner membrane.

FUNCTION

In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB), and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations are associated with juvenile encephalopathy as well as Leigh Syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.