PRODUCT CODE: ET1602-32

Recombinant Scavenging Receptor SR-BI Monoclonal Antibody (ET1602-32)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Scavenging Receptor SR-BI on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-32, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: mouse liver tissue lysate<br />
 Lane 2: human liver tissue lysate
  • Western blot analysis of Scavenging Receptor SR-BI on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-32, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: mouse liver tissue lysate<br />
 Lane 2: human liver tissue lysate
  • ICC staining of Scavenging Receptor SR-BI in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Scavenging Receptor SR-BI in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-32, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Scavenging Receptor SR-BI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-32, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Scavenging Receptor SR-BI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-32, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Scavenging Receptor SR-BI antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-32, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Scavenging Receptor SR-BI on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-32, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse liver tissue lysate
Lane 2: human liver tissue lysate

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Scavenging Receptor SR-BI Monoclonal Antibody (ET1602-32)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse liver tissue lysate, human liver tissue lysate, CRC, PC-12, human liver tissue, human spleen tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR37-06

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

80 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Scavenging Receptor SR-BI

SYNONYMS

CD36 and LIMPII analogous 1 antibody; CD36 antibody; CD36 Antigen like 1 antibody; CD36 antigen-like 1 antibody; CD36L1 antibody; CLA 1 antibody; CLA-1 antibody; CLA1 antibody; Collagen type I receptor antibody; HDLQTL6 antibody; MGC138242 antibody; SCARB1 antibody; Scavebger Receptor Class B Member 1 antibody; Scavenger receptor class B member 1 antibody; Scavenger Receptor Class B Type 1 antibody; SCRB1_HUMAN antibody; SR BI antibody; SR-BI antibody; SRB1 antibody; SRBI antibody; Thrombospondin receptor like 1 antibody; thrombospondin receptor-like 1 antibody

SEQUENCE SIMILARITIES

Belongs to the CD36 family.

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.; The six cysteines of the extracellular domain are all involved in intramolecular disulfide bonds.

SUBCELLULAR LOCATION

Cell membrane, caveola.

FUNCTION

The macrophage class A scavenger receptors (SR-A) type I and II mediate the uptake of modified low density lipoprotein (LDL), while the scavenger receptor class B type 1 (SR-B1) mediates the selective uptake of cholesterol and cholesterol esters (CE) from HDLs into cells. SREC, Ox-LDL-R1, SR-A and SR-B1 may all be involved in the early development of atherosclerosis. SR-B1, an integral membrane protein, acts as a receptor for various ligands, including apoptotic cells, cholesterol ester, phospholipids, lipoproteins and phosphatidyl-serine. SR-B1, which may be involved in phagocytosis of apoptotic cells, enables the movement of cholesterol between the cell surface and extracellular donors and acceptors. Although it is widely expressed, SR-B1 localizes primarily to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.