PRODUCT CODE: ET1706-24

Recombinant SAMHD1 Monoclonal Antibody (ET1706-24)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of SAMHD1 on THP-1 cell lysate using anti-SAMHD1 antibody at 1/1,000 dilution.
  • Western blot analysis of SAMHD1 on THP-1 cell lysate using anti-SAMHD1 antibody at 1/1,000 dilution.
  • ICC staining SAMHD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SAMHD1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining SAMHD1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with SAMHD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of SAMHD1 on THP-1 cell lysate using anti-SAMHD1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant SAMHD1 Monoclonal Antibody (ET1706-24)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

THP-1, HepG2, HUVEC, LOVO, human tonsil tissue, human colon cancer tissue, human spleen tissue, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU56-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

72 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

SAMHD1

SYNONYMS

CHBL2 antibody; DCIP antibody; Dendritic cell derived IFNG induced protein antibody; Dendritic cell-derived IFNG-induced protein antibody; Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody; dNTPase antibody; HD domain containing 1 antibody; HDDC1 antibody; Mg11 antibody; Monocyte protein 5 antibody; MOP 5 antibody; MOP-5 antibody; MOP5 antibody; OTTHUMP00000030889 antibody; SAM domain and HD domain 1 antibody; SAM domain and HD domain containing protein 1 antibody; SAM domain and HD domain-containing protein 1 antibody; SAMH1_HUMAN antibody; Samhd1 antibody; SBBI88 antibody

SEQUENCE SIMILARITIES

Belongs to the SAMHD1 family.

TISSUE SPECIFICITY

Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Thr-592 by CDK1 acts as a switch to control deoxynucleoside triphosphate (dNTPase)-dependent and -independent functions. Phosphorylation at Thr-592 takes place in cycling cells: it reduces the stability of the homotetramer, impairing the dNTPase activity and subsequent ability to restrict infection by viruses. It also inhibits ability to suppress LINE-1 retrotransposon activity. In contrast, phosphorylation at Thr-592 promotes DNA end resection at stalled replication forks in response to DNA damage.; (Microbial infection) Ubiquitinated following interaction with HIV-2 viral protein Vpx; Vpx promotes interaction and with a DCX (DDB1-CUL4-X-box) E3 ubiquitin ligase, leading to proteasomal degradation.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

SAM domain and HD domain-containing protein 1 is a protein that in humans is encoded by the SAMHD1 gene. SAMHD1 is a cellular enzyme, responsible for blocking replication of HIV in dendritic cells, macrophages and monocytes. It is an enzyme that exhibits phosphohydrolase activity, converting deoxynucleoside triphosphates (dNTPs) to inorganic phosphate (iPPP) and a 2'-deoxynucleoside (i.e. deoxynucleosides without a phosphate group). In doing so, SAMHD1 depletes the pool of dNTPs available to a reverse transcriptase for viral cDNA synthesis and thus prevents viral replication. SAMHD1 has also shown nuclease activity. Although a ribonuclease activity was described to be required for HIV-1 restriction, recent data confirmed that SAMHD1-mediated HIV-1 restriction in cells does not involve ribonuclease activity.