PRODUCT CODE: ET1702-82

Recombinant S100P Monoclonal Antibody (ET1702-82)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

ICC staining S100P in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining S100P in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining S100P in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-S100P antibody. Counter stained with hematoxylin.
ICC staining S100P in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant S100P Monoclonal Antibody (ET1702-82)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, HepG2, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF95-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

10 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

S100P

SYNONYMS

MIG9 antibody; Migration inducing gene 9 antibody; Protein S100-E antibody; Protein S100-P antibody; Protein S100P antibody; S100 calcium binding protein P antibody; S100 calcium-binding protein P antibody; S100 P antibody; S100E antibody; S100P antibody; S100P_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the S-100 family.

TISSUE SPECIFICITY

Detected in all of the tissues except brain, testis and small intestine, expression level is higher in placenta, heart, lung, skeletal muscle, spleen and leukocyte. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm, Cell projection.

FUNCTION

S-100 proteins are small dimeric members of the EF-Hand superfamily that participate in moderating intracellular calcium signals by binding to and regulating specific proteins in a calcium-dependent manner. S-100P is a survival factor that is associated with different types of tumors and can bind and regulate effector proteins. R1881, a synthetic androgen, regulates S-100P expression. S-100P interacts with a receptor for advanced glycation end products (RAGE) and activates it, thereby increasing the rates of cell growth, division, migration and invasion. This suggests that S-100P acts in an auto-crine manner through RAGE to trigger cell proliferation and survival. S-100P may also positively affect anchorage-independent growth to improve tumor formation. S-100P monomers strongly interact with one another, but not with other S-100 polypeptides, suggesting that homodimer formation is necessary for S-100P to function. The S-100P dimers are then stabilized by hydrophobic contacts.