PRODUCT CODE: ET1705-62

Recombinant RRM2 Monoclonal Antibody (ET1705-62)

  • Recombinant

Applications

  • WB

  • IP

  • ICC

  • IF

REACTIVITY

  • Human

Western blot analysis of RRM2 on Hela (1) and HepG2 (2) cell lysate using anti-RRM2 antibody at 1/1,000 dilution.
  • Western blot analysis of RRM2 on Hela (1) and HepG2 (2) cell lysate using anti-RRM2 antibody at 1/1,000 dilution.
Western blot analysis of RRM2 on Hela (1) and HepG2 (2) cell lysate using anti-RRM2 antibody at 1/1,000 dilution.

Applications

  • WB

  • IP

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant RRM2 Monoclonal Antibody (ET1705-62)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM93-43

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:20-1:50 IP

TARGET

UNIPROT #

PROTEIN NAME

RRM2

SYNONYMS

cb111 antibody; chunp6884 antibody; etID309896.20 antibody; R2 antibody; reductase M2 polypeptide variant 1 antibody; reductase M2 polypeptide variant 2 antibody; reductase M2 polypeptide variant 3a antibody; reductase M2 polypeptide variant 3b antibody; reductase M2 polypeptide variant 3c antibody; reductase M2 polypeptide variant 3d antibody; Ribonucleoside-diphosphate reductase subunit M2 antibody; Ribonucleotide reductase M2 antibody; Ribonucleotide reductase M2 polypeptide antibody; Ribonucleotide reductase M2 subunit antibody; ribonucleotide reductase protein r2 class I antibody; Ribonucleotide reductase regulatory subunit M2 antibody; Ribonucleotide reductase small chain antibody; Ribonucleotide reductase small subunit antibody; Ribonucleotide reductase, R2 subunit antibody; RIR2_HUMAN antibody; RR2 antibody; RR2M antibody; RRM2 antibody

SEQUENCE SIMILARITIES

Belongs to the ribonucleoside diphosphate reductase small chain family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Belongs to the ribonucleoside diphosphate reductase small chain family. Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.it have two regulatory sites: the specificity site, which controls substrate specificity, and the activity site which regulates overall catalytic activity. A substrate-binding catalytic site, located on M1, is formed only in the presence of the second subunit M2.