PRODUCT CODE: ET1610-83

Recombinant RPS20 Monoclonal Antibody (ET1610-83)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of RPS20 on different lysates using anti-RPS20 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: A431
  • Western blot analysis of RPS20 on different lysates using anti-RPS20 antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: Hela <br />
Lane 2: A431
  • ICC staining RPS20 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RPS20 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of RPS20 on different lysates using anti-RPS20 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: A431

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant RPS20 Monoclonal Antibody (ET1610-83)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, Hela, B16-F1.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC65-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

13 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

40S ribosomal protein S20

GENE NAME

RPS20

SYNONYMS

RPS20

SEQUENCE SIMILARITIES

Belongs to the universal ribosomal protein uS10 family.

POST-TRANSLATIONAL MODIFICATION

Monoubiquitinated by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide.

SUBCELLULAR LOCATION

Cytoplasm.