PRODUCT CODE: ET1703-82

Recombinant RPE65 Monoclonal Antibody (ET1703-82)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of RPE65 on mouse eyeball cells lysates using anti-RPE65 antibody at 1/500 dilution.
  • Western blot analysis of RPE65 on mouse eyeball cells lysates using anti-RPE65 antibody at 1/500 dilution.
Western blot analysis of RPE65 on mouse eyeball cells lysates using anti-RPE65 antibody at 1/500 dilution.

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant RPE65 Monoclonal Antibody (ET1703-82)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse eyeball, rat eyeball cells lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM61-51

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

61 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Retinoid isomerohydrolase

GENE NAME

RPE65

SYNONYMS

RPE65

SEQUENCE SIMILARITIES

Belongs to the carotenoid oxygenase family.

TISSUE SPECIFICITY

Retina (at protein level). Retinal pigment epithelium specific.

POST-TRANSLATIONAL MODIFICATION

Palmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A) (By similarity).

SUBCELLULAR LOCATION

Cytoplasm; Lipid-anchor. Microsome membrane.

FUNCTION

Critical isomerohydrolase in the retinoid cycle involved in regeneration of 11-cis-retinal, the chromophore of rod and cone opsins. Catalyzes the cleavage and isomerization of all-trans-retinyl fatty acid esters to 11-cis-retinol which is further oxidized by 11-cis retinol dehydrogenase to 11-cis-retinal for use as visual chromophore. Essential for the production of 11-cis retinal for both rod and cone photoreceptors. Also capable of catalyzing the isomerization of lutein to meso-zeaxanthin an eye-specific carotenoid. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT (By similarity).