PRODUCT CODE: ET1604-4

Recombinant RNF20 Monoclonal Antibody (ET1604-4)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of RNF20 on different lysates using anti-RNF20 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: MCF-7 <br />
 Lane 3: Jurkat
  • Western blot analysis of RNF20 on different lysates using anti-RNF20 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: MCF-7 <br />
 Lane 3: Jurkat
  • Flow cytometric analysis of K562 cells with RNF20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of RNF20 on different lysates using anti-RNF20 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7
Lane 3: Jurkat

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant RNF20 Monoclonal Antibody (ET1604-4)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, MCF-7, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SP00-47

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

114 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

RNF20

SYNONYMS

BRE1 A antibody; BRE1 antibody; BRE1 E3 ubiquitin ligase homolog antibody; BRE1-A antibody; BRE1A antibody; BRE1A_HUMAN antibody; E3 ubiquitin-protein ligase BRE1A antibody; hBRE1 antibody; Homolog of S. cerevisiae BRE1 antibody; RING finger protein 20 antibody; Ring finger protein 20 E3 ubiquitin protein ligase antibody; Rnf20 antibody

SEQUENCE SIMILARITIES

Belongs to the BRE1 family.

TISSUE SPECIFICITY

Expressed in the normal brain and also in malignant gliomas (at protein level).

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF20 (ring finger protein 20), also known as BRE1, BRE1A or hBRE1, is a 975 amino acid nuclear protein that belongs to the BRE1 family. As a component of the RNF20/40 complex, RNF20 functions as an E3 ubiquitin-protein ligase that regulates the monoubiquitination and subsequent degradation of select residues on target proteins, such as Histone H2B. RNF20 is required for transcriptional activation of Hox genes and is most likely recruited by p53 to the MDM2 promoter, thereby acting as a transcriptional co-activator. RNF20 contains one zinc finger domain and exists as a homodimer.