PRODUCT CODE: ET7107-07

Recombinant RING2 Monoclonal Antibody (ET7107-07)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of RING2 on k562 cell using anti-RING2 antibody at 1/500 dilution.
  • Western blot analysis of RING2 on k562 cell using anti-RING2 antibody at 1/500 dilution.
  •  Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-RING2 antibody. Counter stained with hematoxylin.
Western blot analysis of RING2 on k562 cell using anti-RING2 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant RING2 Monoclonal Antibody (ET7107-07)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, human placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB38-41

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

E3 ubiquitin-protein ligase RING2

GENE NAME

RNF2

SYNONYMS

HIP2-interacting protein 3, RING1b, RNF2, BAP1, DING, HIPI3, RING1B

POST-TRANSLATIONAL MODIFICATION

Polyubiquitinated in the presence of UBE2D3 (in vitro).; Monoubiquitinated, by auto-ubiquitination.

SUBCELLULAR LOCATION

Nucleus. Chromosome. Note=Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells.

FUNCTION

E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation (By similarity). Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (Probable). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes (By similarity).