PRODUCT CODE: ET1704-12

Recombinant Rhodopsin Monoclonal Antibody (ET1704-12)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

Immunohistochemical analysis of paraffin-embedded rat retina tissue using anti-Rhodopsin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat retina tissue using anti-Rhodopsin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat retina tissue using anti-Rhodopsin antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Rhodopsin Monoclonal Antibody (ET1704-12)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat retina tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA10-91

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

39 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Rhodopsin

SYNONYMS

CSNBAD1 antibody; MGC138309 antibody; MGC138311 antibody; OPN 2 antibody; OPN2 antibody; opsd antibody; OPSD_HUMAN antibody; opsin 2 antibody; Opsin 2 rod pigment antibody; Opsin-2 antibody; Opsin2 antibody; Retinitis Pigmentosa 4 antibody; Retinitis pigmentosa 4 autosomal dominant antibody; RHO antibody; Rhodopsin antibody; RP 4 antibody; RP4 antibody

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

TISSUE SPECIFICITY

Rod shaped photoreceptor cells which mediate vision in dim light.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region (By similarity). After activation by light, phosphorylated by GRK1 (in vitro).; Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal(PubMed:12566452).

SUBCELLULAR LOCATION

Membrane.

FUNCTION

In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB), and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations are associated with juvenile encephalopathy as well as Leigh Syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.