PRODUCT CODE: ET1602-23

Recombinant Rho A+B+C Monoclonal Antibody (ET1602-23)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Rho A+B+C on different lysates using anti-Rho A+B+C antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: HepG2 <br />
  Lane 2: Hela <br />
  Lane 3: Jurkat
  • Western blot analysis of Rho A+B+C on different lysates using anti-Rho A+B+C antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: HepG2 <br />
  Lane 2: Hela <br />
  Lane 3: Jurkat
  • ICC staining Rho A+B+C in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Rho A+B+C in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Rho A+B+C in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Rho A+B+C antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Rho A+B+C on different lysates using anti-Rho A+B+C antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Hela
Lane 3: Jurkat

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Rho A+B+C Monoclonal Antibody (ET1602-23)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, NIH/3T3, Hela, HepG2, mouse stomach tissue, human gastric carcinoma tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR38-00

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:200-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Rho A+B+C

SYNONYMS

AA017882 antibody; AI324259, antibody; Aplysia ras related homolog 9 (RhoC) antibody; Aplysia ras-related homolog 12 antibody; Aplysia RAS-related homolog 6 antibody; Aplysia RAS-related homolog antibody; ARH12 antibody; ARH6 antibody; ARH9 antibody; ARHA antibody; ARHB antibody; ARHC antibody; H12 antibody; H6 antibody; H9 antibody; MGC117867 antibody; MGC1448 antibody; MGC61427 antibody; MST081 antibody; MSTP081 antibody; Oncogene RHO H12 antibody; Oncogene RHO H6 antibody; Oncogene RHO H9 antibody; OTTHUMP00000013675 antibody; OTTHUMP00000013676 antibody; OTTHUMP00000013802 antibody; OTTHUMP00000013805 antibody; OTTHUMP00000013807 antibody; OTTHUMP00000013809 antibody; OTTMUSP00000025086 antibody; OTTMUSP00000025087 antibody; Ras homolog 9 (RhoC) antibody; Ras homolog gene family, member A antibody; Ras homolog gene family, member B antibody; Ras homolog gene family, member C antibody; RAS-related homolog 9 antibody; Rh -related GTP binding protein RhoC antibody; Rho cDNA clone 12 antibody; rho cDNA clone 6 antibody; rho cDNA clone 9 antibody; Rho related GTP binding protein RhoB antibody; Rho related GTP binding protein RhoC antibody; RHO12 antibody; RHOA antibody; RHOB antibody; RHOC antibody; rhoC GTPase antibody; RHOH12 antibody; RHOH6 antibody; RHOH9 antibody; RP11-426L16.4 antibody; small GTP binding protein RhoA antibody; Small GTP binding protein RhoC antibody; Transforming protein RhoA antibody

SEQUENCE SIMILARITIES

Belongs to the small GTPase superfamily. Rho family.

POST-TRANSLATIONAL MODIFICATION

(Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Cell membrane, Cleavage furrow.

FUNCTION

The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.