PRODUCT CODE: ET1602-23

Recombinant Rho A+B+C Monoclonal Antibody (ET1602-23)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Rho A+B+C on different lysates using anti-Rho A+B+C antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: HepG2 <br />
 Lane 2: Hela <br />
 Lane 3: Jurkat
  • Western blot analysis of Rho A+B+C on different lysates using anti-Rho A+B+C antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: HepG2 <br />
 Lane 2: Hela <br />
 Lane 3: Jurkat
  • ICC staining Rho A+B+C in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Rho A+B+C in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Rho A+B+C in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Rho A+B+C antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Rho A+B+C antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Rho A+B+C on different lysates using anti-Rho A+B+C antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Hela
Lane 3: Jurkat

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Rho A+B+C Monoclonal Antibody (ET1602-23)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, NIH/3T3, Hela, HepG2, mouse stomach tissue, human gastric carcinoma tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR38-00

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:200-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Rho-related GTP-binding protein RhoC

GENE NAME

RHOC

SYNONYMS

h12, h6, h9

SEQUENCE SIMILARITIES

Belongs to the small GTPase superfamily. Rho family.

POST-TRANSLATIONAL MODIFICATION

(Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly.

SUBCELLULAR LOCATION

Cell membrane. Note=Translocates to the equatorial region before furrow formation in a ECT2-dependent manner.

FUNCTION

Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells.