PRODUCT CODE: ET1611-98

Recombinant Ret Monoclonal Antibody (ET1611-98)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Ret on MCF-7 cells lysates using anti-Ret antibody at 1/1,000 dilution.
  • Western blot analysis of Ret on MCF-7 cells lysates using anti-Ret antibody at 1/1,000 dilution.
  • ICC staining Ret in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Ret in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Ret in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Ret antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Ret antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SW480 cells with Ret antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Ret on MCF-7 cells lysates using anti-Ret antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Ret Monoclonal Antibody (ET1611-98)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7 cell lysates, AGS, MCF-7, SW480, human stomach carcinoma tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN20-28

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

150/175 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Ret

SYNONYMS

C ret antibody; Cadherin family member 12 antibody; Cadherin related family member 16 antibody; CDHF 12 antibody; CDHF12 antibody; CDHR16 antibody; ELKS Fusion gene antibody; HSCR 1 antibody; HSCR1 antibody; Hydroxyaryl protein kinase antibody; MEN2A antibody; MEN2B antibody; MTC 1 antibody; MTC1 antibody; Multiple endocrine neoplasia and medullary thyroid carcinoma 1 antibody; Oncogene RET antibody; Proto oncogene tyrosine protein kinase receptor ret antibody; Proto-oncogene c-Ret antibody; Proto-oncogene tyrosine-protein kinase receptor ret antibody; PTC antibody; RET antibody; RET ELE1 antibody; Ret Proto oncogene antibody; RET transforming sequence antibody; RET_HUMAN antibody; RET51 antibody; RET9 antibody; tyrosine-protein kinase receptor ret antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation. Dephosphorylated by PTPRJ on Tyr-905, Tyr-1015 and Tyr-1062.; Proteolytically cleaved by caspase-3. The soluble RET kinase fragment is able to induce cell death. The extracellular cell-membrane anchored RET cadherin fragment accelerates cell adhesion in sympathetic neurons.

SUBCELLULAR LOCATION

Cell membrane, Endosome membrane.

FUNCTION

The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicomponent receptor complex in conjunction with other membrane-bound, ligand-binding GDNF family receptors. Ligands that bind the Ret receptor include the glial cell line-derived neurotrophic factor (GDNF) and its congeners neurturin, persephin, and artemin. Research studies have shown that alterations in the corresponding RET gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung’s disease. The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr at position 905 dramatically inhibits Ret autophosphorylation activity.