Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Ret Monoclonal Antibody (ET1611-98)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
MCF-7 cell lysates, AGS, MCF-7, SW480, human stomach carcinoma tissue, mouse testis tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN20-28
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
150/175 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:100-1:500
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Ret
SYNONYMS
C ret antibody; Cadherin family member 12 antibody; Cadherin related family member 16 antibody; CDHF 12 antibody; CDHF12 antibody; CDHR16 antibody; ELKS Fusion gene antibody; HSCR 1 antibody; HSCR1 antibody; Hydroxyaryl protein kinase antibody; MEN2A antibody; MEN2B antibody; MTC 1 antibody; MTC1 antibody; Multiple endocrine neoplasia and medullary thyroid carcinoma 1 antibody; Oncogene RET antibody; Proto oncogene tyrosine protein kinase receptor ret antibody; Proto-oncogene c-Ret antibody; Proto-oncogene tyrosine-protein kinase receptor ret antibody; PTC antibody; RET antibody; RET ELE1 antibody; Ret Proto oncogene antibody; RET transforming sequence antibody; RET_HUMAN antibody; RET51 antibody; RET9 antibody; tyrosine-protein kinase receptor ret antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. Tyr protein kinase family.
POST-TRANSLATIONAL MODIFICATION
Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation. Dephosphorylated by PTPRJ on Tyr-905, Tyr-1015 and Tyr-1062.; Proteolytically cleaved by caspase-3. The soluble RET kinase fragment is able to induce cell death. The extracellular cell-membrane anchored RET cadherin fragment accelerates cell adhesion in sympathetic neurons.
SUBCELLULAR LOCATION
Cell membrane, Endosome membrane.
FUNCTION
The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicomponent receptor complex in conjunction with other membrane-bound, ligand-binding GDNF family receptors. Ligands that bind the Ret receptor include the glial cell line-derived neurotrophic factor (GDNF) and its congeners neurturin, persephin, and artemin. Research studies have shown that alterations in the corresponding RET gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung’s disease. The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr at position 905 dramatically inhibits Ret autophosphorylation activity.