PRODUCT CODE: ET1704-98

Recombinant Reelin Monoclonal Antibody (ET1704-98)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Reelin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Reelin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Reelin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Reelin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Reelin antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Reelin Monoclonal Antibody (ET1704-98)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human brain tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA63-13

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

388 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Reelin

SYNONYMS

LIS2 antibody; PRO1598 antibody; Reeler antibody; Reelin antibody; RELN antibody; RELN_HUMAN antibody; RL antibody

SEQUENCE SIMILARITIES

Belongs to the reelin family.

TISSUE SPECIFICITY

Abundantly produced during brain ontogenesis by the Cajal-Retzius cells and other pioneer neurons located in the telencephalic marginal zone and by granule cells of the external granular layer of the cerebellum. In adult brain, preferentially expressed in GABAergic interneurons of prefrontal cortices, temporal cortex, hippocampus and glutamatergic granule cells of cerebellum. Expression is reduced to about 50% in patients with schizophrenia. Also expressed in fetal and adult liver.

DEVELOPMENTAL STAGE

Expressed in fetal and postnatal brain and liver. Expression in postnatal human brain is high in the cerebellum.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Reelin (or Reln) is a large glycoprotein that is secreted by Cajal-Retzius cells in the forebrain and by granule neurons in the cerebellum. Reelin was shown to be mutated in "reeler" mice, a mutation that is associated with widespread disruption of laminated regions of the brain, leading to impaired motor coordination, tremors and ataxia. Reelin protein expression is complex and changes throughout development. Reelin appears to function upstream of Dab1 in a signaling pathway that controls cell positioning in the developing brain and is also thought to be a direct effector of the neurotrophin BDNF.