PRODUCT CODE: ET7107-22

Recombinant RBPJK Monoclonal Antibody (ET7107-22)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

Western blot analysis of RBPJK on human colon tissue lysates using anti-RBPJK antibody at 1/500 dilution.
  • Western blot analysis of RBPJK on human colon tissue lysates using anti-RBPJK antibody at 1/500 dilution.
Western blot analysis of RBPJK on human colon tissue lysates using anti-RBPJK antibody at 1/500 dilution.

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant RBPJK Monoclonal Antibody (ET7107-22)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human colon tissue lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB49-34

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

TARGET

UNIPROT #

PROTEIN NAME

Recombining binding protein suppressor of hairless

GENE NAME

RBPJ

SYNONYMS

RBP-J, RBP-JK, RBPJ, IGKJRB, IGKJRB1, RBPJK, RBPSUH

SEQUENCE SIMILARITIES

Belongs to the Su(H) family.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Note=Mainly nuclear, upon interaction with RITA/C12orf52, translocates to the cytoplasm, down-regulating the Notch signaling pathway.

FUNCTION

Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by repressing transcription of NADPH oxidase subunits (By similarity).