PRODUCT CODE: ET1706-54

Recombinant RbAP48 Monoclonal Antibody (ET1706-54)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: NIH-3T3 <br />
 Lane 2: Hela<br />
 Lane 3: Rat brain <br />
 Lane 4: Mouse testis<br />
  • Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: NIH-3T3 <br />
 Lane 2: Hela<br />
 Lane 3: Rat brain <br />
 Lane 4: Mouse testis<br />
  • ICC staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RbAP48 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RbAP48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.
Positive control:
Lane 1: NIH-3T3
Lane 2: Hela
Lane 3: Rat brain
Lane 4: Mouse testis

Applications

  • WB

  • ICC

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant RbAP48 Monoclonal Antibody (ET1706-54)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH-3T3, Hela, rat brain tissue, mouse testis tissue, MCF-7, SH-SY5Y, human tonsil tissue, human lung cancer tissue, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU37-47

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP:1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Histone-binding protein RBBP4

GENE NAME

RBBP4

SYNONYMS

CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, RBBP-4, RBBP4, RBAP48

SEQUENCE SIMILARITIES

Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.