PRODUCT CODE: ET1706-54

Recombinant RbAP48 Monoclonal Antibody (ET1706-54)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: NIH-3T3 <br />
  Lane 2: Hela<br />
  Lane 3: Rat brain <br />
  Lane 4: Mouse testis
  • Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: NIH-3T3 <br />
  Lane 2: Hela<br />
  Lane 3: Rat brain <br />
  Lane 4: Mouse testis
  • ICC staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RbAP48 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RbAP48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.
Positive control:
Lane 1: NIH-3T3
Lane 2: Hela
Lane 3: Rat brain
Lane 4: Mouse testis

Applications

  • WB

  • ICC

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant RbAP48 Monoclonal Antibody (ET1706-54)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH-3T3, Hela, rat brain tissue, mouse testis tissue, MCF-7, SH-SY5Y, human tonsil tissue, human lung cancer tissue, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU37-47

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP:1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

RbAP48

SYNONYMS

CAF I p48 antibody; CAF-1 subunit C antibody; CAF-I 48 kDa subunit antibody; CAF-I p48 antibody; Chromatin assembly factor 1 subunit C antibody; Chromatin assembly factor I p48 subunit antibody; Chromatin assembly factor/CAF 1 p48 subunit antibody; Histone-binding protein RBBP4 antibody; MSI1 protein homolog antibody; Nucleosome-remodeling factor subunit RBAP48 antibody; NURF55 antibody; RB binding protein 4 chromatin remodeling factor antibody; RbAp 48 antibody; RBAP48 antibody; RBBP-4 antibody; RBBP4 antibody; RBBP4_HUMAN antibody; Retinoblastoma binding protein 4 antibody; Retinoblastoma binding protein p48 antibody; Retinoblastoma-binding protein 4 antibody; Retinoblastoma-binding protein p48 antibody

SEQUENCE SIMILARITIES

Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

In the intact cell, DNA is closely associated with histones and other nuclear proteins to form chromatin.The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation, and an increased accessiblity of DNA to transcription factors. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. The retinoblastoma binding proteins RbAp46 and RbAp48 have been identified as histone binding proteins, and they are components of the histone deacetylase complex.