Positive control:
Lane 1: 293T cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Hela cell lysate
Lane 4: zebrafish tissue lysate
Applications
-
WB
-
ICC
-
IF
-
IP
REACTIVITY
-
Human
-
Mouse
-
Rat
-
Zebrafish
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant Ras Monoclonal Antibody (ET1702-94)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
293T cell lysate, MCF-7 cell lysate, Hela cell lysate, zebrafish tissue lysate, Hela, MCF-7, PC-12.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF10-11
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
21 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:100-1:500
TARGET
UNIPROT #
PROTEIN NAME
Ras
SYNONYMS
C-BAS/HAS antibody; c-H-ras antibody; C-HA-RAS1 antibody; CTLO antibody; GTPase HRas antibody; GTPase KRas antibody; GTPase NRas antibody; H-Ras-1 antibody; H-RASIDX antibody; Ha-Ras antibody; HAMSV antibody; HRAS antibody; HRAS1 antibody; K RAS2A antibody; K RAS2B antibody; K RAS4A antibody; K RAS4B antibody; K-RAS antibody; KRAS antibody; KRAS1 antibody; KRAS2 antibody; N-RAS antibody; N-terminally processed antibody; NRAS antibody; NRAS1 antibody; p21ras antibody; RASH_HUMAN antibody; RASH1 antibody; RASK2 antibody; Transforming protein p21 antibody; v Ha ras Harvey rat sarcoma viral oncogene homolog antibody; v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody; v ras neuroblastoma RAS viral oncogene homolog antibody
SEQUENCE SIMILARITIES
Belongs to the small GTPase superfamily. Ras family.
POST-TRANSLATIONAL MODIFICATION
Palmitoylated by the ZDHHC9-GOLGA7 complex. Depalmitoylated by ABHD17A, ABHD17B and ABHD17C. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.; Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).; Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.; Phosphorylation at Ser-89 by STK19 enhances NRAS-association with its downstream effectors.
SUBCELLULAR LOCATION
Cytoplasm, Cell membrane, Golgi apparatus, Nucleus.
FUNCTION
The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode guanine nucleotide-binding proteins that are ubiquitously expressed in vertebrate cells. c-H- and c-K-Ras are cellular homologs of the v-H and v-K-Ras sequences originally isolated from the Harvey and Kirsten strains of rat sarcoma virus. Ras-encoded proteins bind GDP and GTP with high affinity and possess a low level intrinsic GTPase activity that can be stimulated over 100-fold by interaction with cytosolic GTPase activating protein (GAP), a potential effector for Ras p21 function. Point mutations at amino acids 12, 13, 59 and 61 within domains responsible for GTP binding and hydrolysis activate Ras proteins to their oncogenic form and block the ability of the GTPase activity to be stimulated by GAP. Several additional proteins with GAP activity have been identified and shown to interact with p21 Ras or other members of the Ras gene family.