PRODUCT CODE: ET1705-70

Recombinant RANTES Monoclonal Antibody (ET1705-70)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of RANTES on mouse thymus tissue lysate using anti-RANTES antibody at 1/1,000 dilution.
  • Western blot analysis of RANTES on mouse thymus tissue lysate using anti-RANTES antibody at 1/1,000 dilution.
  • ICC staining RANTES in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RANTES in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RANTES antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-RANTES antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung caner tissue using anti-RANTES antibody. Counter stained with hematoxylin.
Western blot analysis of RANTES on mouse thymus tissue lysate using anti-RANTES antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant RANTES Monoclonal Antibody (ET1705-70)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse thymus tissue lysate, Hela, HUVEC, human tonsil tissue, human breast cancer tissue, human lung caner tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM03-45

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

10 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

C-C motif chemokine 5

GENE NAME

CCL5

SYNONYMS

TCP228, CCL5, D17S136E, SCYA5

SEQUENCE SIMILARITIES

Belongs to the intercrine beta (chemokine CC) family.

TISSUE SPECIFICITY

Expressed in the follicular fluid (at protein level). T-cell and macrophage specific.

POST-TRANSLATIONAL MODIFICATION

N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.; The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.