PRODUCT CODE: ET1610-88

Recombinant RALBP1 Monoclonal Antibody (ET1610-88)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of RALBP1 on different lysates using anti-RALBP1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: HCT116 <br />
 Lane 2: Jurkat <br />
 Lane 3: 293T
  • Western blot analysis of RALBP1 on different lysates using anti-RALBP1 antibody at 1/1,000 dilution.<br />
Positive control: <br />
 Lane 1: HCT116 <br />
 Lane 2: Jurkat <br />
 Lane 3: 293T
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293 cells with RALBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of RALBP1 on different lysates using anti-RALBP1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HCT116
Lane 2: Jurkat
Lane 3: 293T

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant RALBP1 Monoclonal Antibody (ET1610-88)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HCT116, Jurkat, human breast carcinoma tissue, human pancreas tissue, mouse lung tissue, mouse pancreas tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC06-49

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

95 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

RalA-binding protein 1

GENE NAME

RALBP1

SYNONYMS

DNP-SG ATPase, RALBP1

TISSUE SPECIFICITY

Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.

SUBCELLULAR LOCATION

Membrane; Peripheral membrane protein.

FUNCTION

Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.