PRODUCT CODE: ET1702-27

Recombinant RAGE Monoclonal Antibody (ET1702-27)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of RAGE on mouse lung lysates using anti-RAGE antibody at 1/1,000 dilution.
  • Western blot analysis of RAGE on mouse lung lysates using anti-RAGE antibody at 1/1,000 dilution.
  •  Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-RAGE antibody. Counter stained with hematoxylin.
  •  Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RAGE antibody. Counter stained with hematoxylin.
Western blot analysis of RAGE on mouse lung lysates using anti-RAGE antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant RAGE Monoclonal Antibody (ET1702-27)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, MCF-7, mouse lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF0975

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Advanced glycosylation end product-specific receptor

GENE NAME

Ager

SYNONYMS

AGER

TISSUE SPECIFICITY

Isoform 1: Expressed at higher levels in the coronary arterioles in type 2 diabetic mice (at protein level). Endothelial cells. Expressed in lung, kidney, brain and heart. Most prevalent isoform with the highest level in heart. Isoform 2: Expressed in brain, lung, kidney and small intestine with the highest level in lung. Expressed in brain, lung, kidney and small intestine with the highest level in small intestine (at protein level). Detected in neurons of the cerebrum, bronchial epithelium, endothelial cells, tubular cells of kidney and epithelial cells of small intestine (at protein level). Expression is increased in the kidney of diabetic wild-type mice (at protein level), but not in the other tissues. Expressed only in kidney. Expression is increased in the kidney of diabetic mice. Isoform 3: Expressed in lung, kidney and heart. The second most prevalent isoform with the highest level in lung. Not expressed in brain. Isoform 4: Expressed at very low level in lung only. Isoform 5: Expressed at very low level in lung only. Isoform 6: Expressed at very low level in lung only. Isoform 7: Expressed at very low level in heart only. Isoform 8: Expressed at very low level in lung only. Isoform 9: Expressed at very low level in heart only. Isoform 10: Expressed in lung, brain, heart and kidney with a very high level in kidney. Isoform 11: Expressed in brain, kidney and heart. Not expressed in lung. Isoform 12: Expressed at very low level in lung and kidney. Isoform 13: Expressed at very low level in lung only.

SUBCELLULAR LOCATION

[Isoform 1]: Membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Isoform 10]: Cell membrane; Single-pass membrane protein.

FUNCTION

Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Can also bind oligonucleotides. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. RAGE-dependent signaling in microglia contributes to neuroinflammation, amyloid accumulation, and impaired learning/memory in a mouse model of Alzheimer disease.; [Isoform 2]: Is able to advanced glycosylation end product (AGE)-induce nuclear factor NF-kappa-B activation.; [Isoform 10]: Down-regulates receptor for advanced glycosylation end products (RAGE)-ligand induced signaling through various MAPK pathways including ERK1/2, p38 and SAPK/JNK. Significantly affects tumor cell properties through decreasing cell migration, invasion, adhesion and proliferation, and increasing cellular apoptosis. Exhibits drastic inhibition on tumorigenesis in vitro.