PRODUCT CODE: ET1611-32

Recombinant RAD18 Monoclonal Antibody (ET1611-32)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: NIH/3T3 <br />
 Lane 2: 293T <br />
 Lane 3: MCF-7
  • Western blot analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: NIH/3T3 <br />
 Lane 2: 293T <br />
 Lane 3: MCF-7
  • ICC staining RAD18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RAD18 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining RAD18 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RAD18 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with RAD18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1,000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: 293T
Lane 3: MCF-7

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant RAD18 Monoclonal Antibody (ET1611-32)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

NIH/3T3, MCF-7, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN66-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

56/90kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

RAD18

SYNONYMS

2810024C04Rik antibody; DNA repair protein rad18 antibody; E3 ubiquitin-protein ligase RAD18 antibody; EC 6.3.2.- antibody; hHR 18 antibody; hHR18 antibody; hRAD 18 antibody; hRAD18 antibody; MGC156682 antibody; Postreplication repair E3 ubiquitin-protein ligase RAD18 antibody; Postreplication repair protein hRAD18p antibody; Postreplication repair protein RAD18 antibody; RAD 18 antibody; RAD18 antibody; RAD18 homolog (S. cerevisiae) antibody; RAD18 homolog antibody; RAD18 S. cerevisiae homolog antibody; RAD18 S. cerevisiae homolog of antibody; RAD18 transcript variant antibody; RAD18_HUMAN antibody; Rad18sc antibody; Radiation sensitivity protein 18 antibody; RING finger protein 73 antibody; RNF 73 antibody; RNF73 antibody; Structural maintenance of chromosomes protein 6 antibody; YCR066W antibody; YCR66W antibody

SEQUENCE SIMILARITIES

Belongs to the RAD18 family.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

The RING-type zinc finger protein Rad18 is essential in post-replication repair of damaged DNA and contributes to the maintenance of genomic stability. Rad18 maintains chromosomal DNA with the Rad54-dependent DNA repair pathway and recruits ubiquitin-conjugating enzymes in the Rad6 pathway. Rad18 functions in gap-filling of a daughter strand on replication of damaged DNA and is localized in the nucleus.