PRODUCT CODE: ET1602-29

Recombinant Rab9 Monoclonal Antibody (ET1602-29)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Rab9 on different lysates using anti-Rab9 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: HepG2 <br />
 Lane 2: K562
  • Western blot analysis of Rab9 on different lysates using anti-Rab9 antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: HepG2 <br />
 Lane 2: K562
  • ICC staining Rab9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Rab9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of Rab9 on different lysates using anti-Rab9 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: K562

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Rab9 Monoclonal Antibody (ET1602-29)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, Hela, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR45-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

23 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Rab9

SYNONYMS

2410064E05Rik antibody; AI195561 antibody; DmRab9 antibody; Rab 9 antibody; RAB 9A antibody; RAB9 member RAS oncogene family antibody; RAB9A antibody; RAB9A member RAS oncogene family antibody; RAB9A_HUMAN antibody; RAS ASSOCIATED PROTEIN RAB9 antibody; Ras related protein Rab 9A antibody; Ras-related protein Rab-9A antibody; Sid6061p antibody; Sid99 antibody

SEQUENCE SIMILARITIES

Belongs to the small GTPase superfamily. Rab family.

SUBCELLULAR LOCATION

Cell membrane, Endoplasmic reticulum membrane, Golgi apparatus membrane.

FUNCTION

The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.