PRODUCT CODE: ET1602-15

Recombinant Rab4A Monoclonal Antibody (ET1602-15)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Rat

  • Monkey

  • Mouse

Western blot analysis of Rab4A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: MCF-7 cell lysate<br />
Lane 2: 293T cell lysate<br />
Lane 3: COS-1 cell lysate
  • Western blot analysis of Rab4A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: MCF-7 cell lysate<br />
Lane 2: 293T cell lysate<br />
Lane 3: COS-1 cell lysate
  • ICC staining of Rab4A in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of Rab4A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: 293T cell lysate
Lane 3: COS-1 cell lysate

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Rat

  • Monkey

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Rab4A Monoclonal Antibody (ET1602-15)

Immunogen

Synthetic peptide within human rab4a aa 180-218.

Host

Rabbit

Positive Control

MCF-7 cell lysate, 293T cell lysate, COS-1 cell lysate, PC-12.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR27-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IP

  • assay-dependent

TARGET

UNIPROT #

PROTEIN NAME

Ras-related protein Rab-4A

GENE NAME

RAB4A

SYNONYMS

RAB4A

SEQUENCE SIMILARITIES

Belongs to the small GTPase superfamily. Rab family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by CDK1 kinase during mitosis.

SUBCELLULAR LOCATION

Membrane; Peripheral membrane protein. Cytoplasm. Early endosome membrane.

FUNCTION

Protein transport. Plays a role in vesicular traffic. Mediates VEGFR2 endosomal trafficking to enhance VEGFR2 signaling.