PRODUCT CODE: ET1602-15

Recombinant Rab4A Monoclonal Antibody (ET1602-15)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Rat

  • Monkey

  • Mouse

Western blot analysis of Rab4A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: MCF-7 cell lysate<br />
 Lane 2: 293T cell lysate<br />
 Lane 3: COS-1 cell lysate
  • Western blot analysis of Rab4A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: MCF-7 cell lysate<br />
 Lane 2: 293T cell lysate<br />
 Lane 3: COS-1 cell lysate
  • ICC staining of Rab4A in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-15, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of Rab4A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: 293T cell lysate
Lane 3: COS-1 cell lysate

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

  • Rat

  • Monkey

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Rab4A Monoclonal Antibody (ET1602-15)

Immunogen

Synthetic peptide within human rab4a aa 180-218.

Host

Rabbit

Positive Control

MCF-7 cell lysate, 293T cell lysate, COS-1 cell lysate, PC-12.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR27-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC/IF

  • 1:50-1:200

  • IP

  • assay-dependent

TARGET

UNIPROT #

PROTEIN NAME

Rab4A

SYNONYMS

HRES 1 / RAB4 antibody; Oncogene RAB4 antibody; Rab 4 antibody; RAB 4A antibody; RAB4 member RAS oncogene family antibody; Rab4a antibody; RAB4A member RAS oncogene family antibody; RAB4A_HUMAN antibody; Ras related protein Rab 4A antibody; Ras related protein Rab4A antibody; Ras-related protein Rab-4A antibody

SEQUENCE SIMILARITIES

Belongs to the small GTPase superfamily. Rab family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by CDK1 kinase during mitosis.

SUBCELLULAR LOCATION

Cytoplasm, Membrane

FUNCTION

The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab superfamilies, exhibits 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possiblity that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab family have been identified, each of which is found at a particular stage of a membrane transport pathway.