PRODUCT CODE: ET7107-49

Recombinant PU.1/Spi1 Monoclonal Antibody (ET7107-49)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of PU.1/Spi1 on THP-1 cell using anti-PU.1/Spi1 antibody at 1/500 dilution.
  • Western blot analysis of PU.1/Spi1 on THP-1 cell using anti-PU.1/Spi1 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PU.1/Spi1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PU.1/Spi1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with PU.1/Spi1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of PU.1/Spi1 on THP-1 cell using anti-PU.1/Spi1 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PU.1/Spi1 Monoclonal Antibody (ET7107-49)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human tonsil tissue, human spleen tissue, K562, THP-1.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB72-35

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

31 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PU.1/Spi1

SYNONYMS

transcription factor spi1 antibody; 31 kDa Transforming Protein antibody; 31 kDa-transforming protein antibody; cb1086 antibody; Hematopoietic transcription factor PU.1 antibody; OF antibody; oncogene spi1 antibody; PU.1 antibody; SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody; SFPI1 antibody; si:by184l24.2 antibody; SPI 1 antibody; SPI 1 proto oncogene antibody; SPI A antibody; Spi1 antibody; SPI1_HUMAN antibody; Spleen focus forming virus (SFFV) proviral integration oncogene spi1 antibody; Spleen focus forming virus proviral integration oncogene spi1 antibody; Transcription factor PU.1 antibody

SEQUENCE SIMILARITIES

Belongs to the ETS family.

SUBCELLULAR LOCATION

Cytosol.

FUNCTION

The Ets transcription factor family (Ets-1, Ets-2, Erg-1-3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF) are DNA-binding proteins that influence lymphoid development and activity. The Ets family monomeric proteins bind the consensus DNA site GGA(A/T) through a unique winged helix-turn-helix motif known as the Ets domain. PU.1 (Spi-1/Spi-A), Spi-B and Spi-C are closely related Ets family members which share a conserved divergent sequence within the Ets domain that enables their binding to the non-canonical AGAA sites. PU.1 transactivates a large number of B cell genes, such as those encoding CD72, CD20 and Btk, and Spi-B enhances expression of many of these same target genes. PU.1 is expressed in a wide variety of hematopoetic cells, including B cells, early T-cells, megakaryocytes, granulocytes, mast cells, immature erythrocytes and myeloid cells. Alternatively, Spi-B expression is limited to B cells and immature T cells, where expression accumulates through T-lineage commitment and then is dramatically absent following the beta-selection checkpoint.