PRODUCT CODE: ET1701-90

Recombinant PTP1B Monoclonal Antibody (ET1701-90)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Zebrafish

Western blot analysis of PTP1B on different lysates using anti-PTP1B antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: zebrafish <br />
Lane 2: MCF-7 <br />
Lane 3: HepG2 <br />
Lane 4: A431
  • Western blot analysis of PTP1B on different lysates using anti-PTP1B antibody at 1/1,000 dilution.<br />
Positive control: <br />
Lane 1: zebrafish <br />
Lane 2: MCF-7 <br />
Lane 3: HepG2 <br />
Lane 4: A431
  • ICC staining PTP1B in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PTP1B in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PTP1B in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Raji cells with PTP1B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of PTP1B on different lysates using anti-PTP1B antibody at 1/1,000 dilution.
Positive control:
Lane 1: zebrafish
Lane 2: MCF-7
Lane 3: HepG2
Lane 4: A431

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PTP1B Monoclonal Antibody (ET1701-90)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, MCF-7, Hela, HepG2, human tonsil tissue, Zebrafish.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0935

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Tyrosine-protein phosphatase non-receptor type 1

GENE NAME

PTPN1

SYNONYMS

PTP-1B

SEQUENCE SIMILARITIES

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.

TISSUE SPECIFICITY

Expressed in keratinocytes (at protein level).

POST-TRANSLATIONAL MODIFICATION

Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.; Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.; S-nitrosylation of Cys-215 inactivates the enzyme activity.; Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Note=Interacts with EPHA3 at the cell membrane.

FUNCTION

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.