PRODUCT CODE: ET1701-90

Recombinant PTP1B Monoclonal Antibody (ET1701-90)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Zebrafish

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Western blot analysis of PTP1B on different lysates using anti-PTP1B antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: zebrafish <br />
 Lane 2: MCF-7 <br />
 Lane 3: HepG2 <br />
 Lane 4: A431
  • Western blot analysis of PTP1B on different lysates using anti-PTP1B antibody at 1/1,000 dilution.<br />
 Positive control: <br />
 Lane 1: zebrafish <br />
 Lane 2: MCF-7 <br />
 Lane 3: HepG2 <br />
 Lane 4: A431
  • ICC staining PTP1B in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PTP1B in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PTP1B in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Raji cells with PTP1B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of PTP1B on different lysates using anti-PTP1B antibody at 1/1,000 dilution.
Positive control:
Lane 1: zebrafish
Lane 2: MCF-7
Lane 3: HepG2
Lane 4: A431

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PTP1B Monoclonal Antibody (ET1701-90)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, MCF-7, Hela, HepG2, human tonsil tissue, Zebrafish.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0935

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PTP1B

SYNONYMS

PTP1B antibody; Non receptor tyrosine phosphatase 1 antibody; Protein phosphotyrosylphosphatase 1B antibody; Protein tyrosine phosphatase 1B antibody; Protein tyrosine phosphatase non receptor type 1 antibody; Protein tyrosine phosphatase placental antibody; Protein-tyrosine phosphatase 1B antibody; PTN1_HUMAN antibody; PTP 1B antibody; PTP-1B antibody; PTPN 1 antibody; PTPN1 antibody; Tyrosine protein phosphatase non receptor type 1 antibody; Tyrosine-protein phosphatase non-receptor type 1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.

TISSUE SPECIFICITY

Expressed in keratinocytes (at protein level).

POST-TRANSLATIONAL MODIFICATION

Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.; Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.; S-nitrosylation of Cys-215 inactivates the enzyme activity.; Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane.

FUNCTION

The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction. This is a reversible process, involving both enzymes that phosphorylate proteins on tyrosine residues as well as a rapidly expanding family of protein tyrosine phosphatases. These latter enzymes bear little resemblance to either the protein serine and protein threonine phosphatases or to the acid and alkaline phosphatases. In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein. On the basis of sequence analysis, PTP1B (PTPase 1B) expressed in human placenta exhibits similarities both with the common leukocyte antigen (CD45) and with LAR, a homolog of the neural adhesion molecule (NCAM). PTP1B is synthesized as a 435 amino acid precursor protein which is cleaved to generate the active 321 amino acid enzyme.