PRODUCT CODE: ET1706-19

Recombinant PSMA1 Monoclonal Antibody (ET1706-19)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PSMA1 on different cell lysates using anti-PSMA1 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: Jurkat<br />
 Lane 2: Hela<br />
 Lane 3: HepG2<br />
 Lane 4: 293<br />
  • Western blot analysis of PSMA1 on different cell lysates using anti-PSMA1 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: Jurkat<br />
 Lane 2: Hela<br />
 Lane 3: HepG2<br />
 Lane 4: 293<br />
  • Western blot analysis of PSMA1 on different lysates using anti-PSMA1 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1: PC-12<br />
 Lane 2: Mouse spleen tissue<br />
 Lane 3: Rat spleen tissue<br />
  • ICC staining PSMA1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PSMA1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.
  • ICC staining PSMA1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.
Western blot analysis of PSMA1 on different cell lysates using anti-PSMA1 antibody at 1/500 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: Hela
Lane 3: HepG2
Lane 4: 293

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PSMA1 Monoclonal Antibody (ET1706-19)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, Hela, HepG2, 293, PC-12, mouse spleen tissue lysate, rat spleen tissue, MCF-7, PC-3M, rat testis tissue, human liver cancer tissue, human pancreas tissue, mouse cerebellum tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU66-24

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

30 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:200-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Proteasome subunit alpha type-1

GENE NAME

PSMA1

SYNONYMS

PROS-30, PSMA1, HC2, NU, PROS30, PSC2

SEQUENCE SIMILARITIES

Belongs to the peptidase T1A family.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).