PRODUCT CODE: ET1610-54

Recombinant PRP19 Monoclonal Antibody (ET1610-54)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PRP19 on Jurkat cells lysates using anti-PRP19 antibody at 1/1,000 dilution.
  • Western blot analysis of PRP19 on Jurkat cells lysates using anti-PRP19 antibody at 1/1,000 dilution.
  • ICC staining PRP19 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PRP19 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PRP19 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRP19 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with PRP19 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of PRP19 on Jurkat cells lysates using anti-PRP19 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PRP19 Monoclonal Antibody (ET1610-54)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, MCF-7, Hela, B16-F1, mouse brain tissue, human lung cancer tissue, mouse kidney tissue, human breast carcinoma tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC06-53

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PRP19

SYNONYMS

hPso4 antibody; NMP200 antibody; Nuclear matrix protein 200 antibody; Nuclear matrix protein NMP200 related to splicing factor PRP19 antibody; pre mRNA processing factor 19 antibody; Pre-mRNA-processing factor 19 antibody; PRP19 antibody; PRP19/PSO4 homolog antibody; PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) antibody; PRP19_HUMAN antibody; PRPF19 antibody; PSO4 antibody; psoralen 4 antibody; Senescence evasion factor antibody; SNEV antibody; UBOX4 antibody

SEQUENCE SIMILARITIES

Belongs to the WD repeat PRP19 family.

TISSUE SPECIFICITY

Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm, Lipid droplet.

FUNCTION

The spliceosome, the 0gigantic molecular machine that performs pre-mRNA splicing in eukaryotes, contains over 200 different proteins and five RNA molecules (U1, U2, U4, U5 and U6). Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The PRP19-associated complex is required for stable association of U5 and U6 with the spliceosome after U4 is released. Changes within the spliceosome upon binding of the PRP19-associated complex include remodeling of the U6/5' splice site interaction and destabilization of Lsm proteins to allow further interaction of U6 with the intron sequence.