PRODUCT CODE: ET7106-87

Recombinant Protein Kinase D2 Monoclonal Antibody (ET7106-87)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Protein Kinase D2 on A549 cell using anti-Protein Kinase D2 antibody at 1/500 dilution.
  • Western blot analysis of Protein Kinase D2 on A549 cell using anti-Protein Kinase D2 antibody at 1/500 dilution.
  • ICC staining Protein Kinase D2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Protein Kinase D2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Protein Kinase D2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY-5Y cells with Protein Kinase D2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Protein Kinase D2 on A549 cell using anti-Protein Kinase D2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Protein Kinase D2 Monoclonal Antibody (ET7106-87)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, SH-SY-5Y, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU08-33

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

97 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Protein Kinase D2

SYNONYMS

DKFZp586E0820 antibody; HSPC187 antibody; KPCD2_HUMAN antibody; nPKC D2 antibody; nPKC-D2 antibody; PKD2 antibody; PRKD 2 antibody; Prkd2 antibody; Protein kinase D2 antibody; Serine/threonine protein kinase D2 antibody; Serine/threonine-protein kinase D2 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of Ser-876 correlates with the activation status of the kinase. Ser-706 or/and Ser-710 are probably phosphorylated by PKC. Phosphorylation at Ser-244 by CSNK1D and CSNK1E promotes nuclear localization and substrate targeting. Phosphorylation at Ser-244, Ser-706 and Ser-710 is required for nuclear localization. Phosphorylated at Tyr-438 by ABL1 in response to oxidative stress. Phosphorylated at Tyr-717 by ABL1 specifically in response to oxidative stress; requires prior phosphorylation at Ser-706 or/and Ser-710.

SUBCELLULAR LOCATION

Membrane. Cytoplasm. Nucleus.

FUNCTION

PKD2 (protein kinase D2), also known as PRKD2 or HSPC187, is a widely expressed protein belonging to the protein kinase D (PKD) family of serine/threonine kinases. In mammals, there are three members of the PKD family, namely PKC µ, PKD2 and PKC n, and each contain a homologous catalytic domain but differ in their tissue expression and subcellular localization. PKD family members are activated by G-protein-coupled receptors (GPCRs) and are known to participate in biological processes such as proliferation, apoptosis, migration, signal transduction and vesicle shedding. Shuttling between the nucleus and the cytoplasm, PKD2 contains one PH domain, one protein kinase domain and two phorbol-ester/DAG-type zinc fingers, and functions as a calcium-independent, phospholipid-dependent protein kinase. Upon activation of CCK-BR, PKD2 is phosphorylated by casein kinase I isoforms and subsequently accumulates in the nucleus. The result of the nuclear accumulation of PKD2 is the transcriptional activation of Nur77 and the nuclear exclusion of HDAC7. This suggests that PKD2 mediates CCK-BR-induced transcriptional activation.