PRODUCT CODE: ET7107-24

Recombinant Proteasome 20S LMP2 Monoclonal Antibody (ET7107-24)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Proteasome 20S LMP2 on different lysates using anti-Proteasome 20S LMP2 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse spleen tissue <br />
  Lane 2: U937 <br />
  Lane 2: Mouse colon tissue
  • Western blot analysis of Proteasome 20S LMP2 on different lysates using anti-Proteasome 20S LMP2 antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: Mouse spleen tissue <br />
  Lane 2: U937 <br />
  Lane 2: Mouse colon tissue
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Proteasome 20S LMP2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Daudi cells with Proteasome 20S LMP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of Proteasome 20S LMP2 on different lysates using anti-Proteasome 20S LMP2 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse spleen tissue
Lane 2: U937
Lane 2: Mouse colon tissue

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Proteasome 20S LMP2 Monoclonal Antibody (ET7107-24)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

U937, mouse colon tissue lysate, human spleen tissue, Daudi.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB35-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

23 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Proteasome 20S LMP2

SYNONYMS

Beta1i antibody; Large multifunctional peptidase 2 antibody; Large multifunctional protease 2 antibody; LMP 2 antibody; LMP2 antibody; Low molecular mass protein 2 antibody; Macropain chain 7 antibody; MGC70470 antibody; Multicatalytic endopeptidase complex chain 7 antibody; OTTHUMP00000062982 antibody; Proteasome (prosome macropain) subunit beta type 9 antibody; proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) antibody; Proteasome beta 9 subunit antibody; Proteasome catalytic subunit 1i antibody; Proteasome chain 7 antibody; Proteasome related gene 2 antibody; Proteasome subunit beta 6i antibody; Proteasome subunit beta type 9 antibody; Proteasome subunit beta type-9 antibody; Proteasome subunit beta-1i antibody; PSB9_HUMAN antibody; PSMB 9 antibody; PSMB6i antibody; PSMB9 antibody; Really interesting new gene 12 protein antibody; RING 12 antibody; RING12 antibody; RING12 protein antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase T1B family.

DEVELOPMENTAL STAGE

Highly expressed in immature dendritic cells (at protein level).

POST-TRANSLATIONAL MODIFICATION

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

The eukaryotic multicatalytic proteinase complex, otherwise known as the proteasome, is present in both the nucleus and cytoplasm of cells and contains at least 15 nonidentical subunits, which form a highly ordered ring-shaped structure. The proteasome is involved in an ATP/Ubiquitin-dependent proteolytic pathway and expresses at least five distinct proteolytic activities, including the cleavage of peptides after branched chain amino acids or bulky hydrophobic amino acids. Two components of the proteasome are the low molecular mass proteins LMP2 and LMP7, which are thought to connect the proteasome to the MHC class-I antigen-processing pathway. Upon stimulation with IFN-γ, LMP2 and LMP7 displace housekeeping subunits in the proteasome and activate cytotoxic T cells (CTLs). LMP2 and LMP7 are produced as precursor proteins, which are processed to subunits that have the ability to complex with the proteasome. LMP2 is expressed as two alternatively spliced forms, LMP2.l and LMP2.s, in lymphoblastoid cell lines and in fibroblasts after IFN-γ stimulation. LMP7 is also expressed as two forms, LMP7-E1 and E2, in several tissues.