PRODUCT CODE: ET1702-57

Recombinant PPAR gamma Monoclonal Antibody (ET1702-57)

  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

-
+
Western blot analysis of PPAR gamma on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: PC-12 cell lysate<br />
 Lane 2: K562 cell lysate
  • Western blot analysis of PPAR gamma on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: PC-12 cell lysate<br />
 Lane 2: K562 cell lysate
Western blot analysis of PPAR gamma on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-57, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: PC-12 cell lysate
Lane 2: K562 cell lysate

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PPAR gamma Monoclonal Antibody (ET1702-57)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12 cell lysate, K562 cell lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF101-4

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

PPAR gamma

SYNONYMS

CIMT1 antibody; GLM1 antibody; NR1C3 antibody; Nuclear receptor subfamily 1 group C member 3 antibody; OTTHUMP00000185032 antibody; OTTHUMP00000185036 antibody; Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody; Peroxisome proliferator activated receptor gamma 1 antibody; Peroxisome Proliferator Activated Receptor gamma antibody; Peroxisome proliferator-activated receptor gamma antibody; PPAR gamma antibody; PPAR-gamma antibody; PPARG antibody; PPARG_HUMAN antibody; PPARG1 antibody; PPARG2 antibody; PPARgamma antibody

SEQUENCE SIMILARITIES

Belongs to the nuclear hormone receptor family. NR1 subfamily.

TISSUE SPECIFICITY

Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.

POST-TRANSLATIONAL MODIFICATION

O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes.; Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation at Ser-112 induces adipogenic activity (By similarity).

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs). These heterodimers regulate transcription of genes involved in insulin action, adipocyte differentiation, lipid metabolism and inflammation. PPARγ is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. PPARγ activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. A key component in adipocyte differentiation and fat-specific gene expression, PPARγ may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake. A Pro12Ala polymorphism of the PPARγ2 gene has been reported to reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPARγ2 gene maybe correlated with abdominal obesity in type 2 diabetes.