Lane 1: A431 cell lysate
Lane 2: 293T cell lysate
Lane 3: NIH/3T3 cell lysate
Recombinant Rabbit monoclonal primary
Recombinant PP2A alpha + beta Monoclonal Antibody (ET1611-54)
A431, NIH/3T3, 293T, PANC-1, Hela, L6, rat kidney tissue, human tonsil tissue, human pancreas tissue, mouse brain tissue, mouse stomach tissue, mouse heart tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
PP2A-alpha, RP-C, PPP2CA, PP2A-beta, PPP2CB
Belongs to the PPP phosphatase family. PP-1 subfamily.
Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.; Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.; May be monoubiquitinated by NOSIP.
Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles.
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.
Liu, Yu et al.
Proteomic Maps of Human Gastrointestinal Stromal Tumor Subgroups. | Molecular & Cellular Proteomics : Mcp