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PRODUCT CODE: ET1705-15

Recombinant PMP22 Monoclonal Antibody (ET1705-15)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

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Western blot analysis of PMP22 on PC-12 cell using anti-PMP22 antibody at 1/500 dilution.
  • Western blot analysis of PMP22 on PC-12 cell using anti-PMP22 antibody at 1/500 dilution.
  • ICC staining PMP22 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PMP22 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with PMP22 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of PMP22 on PC-12 cell using anti-PMP22 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PMP22 Monoclonal Antibody (ET1705-15)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, N2A, SH-SY5Y, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM52-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q01453

PROTEIN NAME

Peripheral myelin protein 22

GENE NAME

PMP22

SYNONYMS

PMP-22, GAS-3, PMP22, GAS3

SEQUENCE SIMILARITIES

Belongs to the PMP-22/EMP/MP20 family.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein.

FUNCTION

Might be involved in growth regulation, and in myelinization in the peripheral nervous system.