PRODUCT CODE: ET1705-15

Recombinant PMP22 Monoclonal Antibody (ET1705-15)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of PMP22 on PC-12 cell using anti-PMP22 antibody at 1/500 dilution.
  • Western blot analysis of PMP22 on PC-12 cell using anti-PMP22 antibody at 1/500 dilution.
  • ICC staining PMP22 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PMP22 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with PMP22 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of PMP22 on PC-12 cell using anti-PMP22 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PMP22 Monoclonal Antibody (ET1705-15)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12 cell lysates, N2A, rat brain tissue, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM52-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PMP22

SYNONYMS

CMT1A antibody; CMT1E antibody; DSS antibody; GAS-3 antibody; GAS3 antibody; Growth Arrest Specific 3 antibody; Growth arrest-specific protein 3 antibody; HMSNIA antibody; HNPP antibody; MGC20769 antibody; Peripheral myelin protein 22 antibody; PMP-22 antibody; PMP22 antibody; PMP22_HUMAN antibody; Sp110 antibody; Trembler antibody

SEQUENCE SIMILARITIES

Belongs to the PMP-22/EMP/MP20 family.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

PLP (myelin proteolipid protein or lipophilin) is a major constituent of myelin. The two isoforms of the myelin proteolipid protein, PLP and DM20, are very hydrophobic integral membrane proteins that account for about half of the protein content of adult CNS myelin. A mutation in the gene which encodes PLP is linked to Pelizaeus-Merzbacher disease (PMD), a chronic infantile type of diffuse cerebral sclerosis. The gene which encodes PLP maps to human chromosome Xq13-q22. The glycoprotein zero (also designated P-zero or myelin peripheral protein) is the major structural protein of peripheral myelin, accounting for more than 50% of the protein present in the sheath of peri-pheral nerves. Zero is an integral membrane glycoprotein whose expression is restricted to Schwann cells. The gene which encodes zero maps to human chromosome 1q22. PMP22 (peripheral myelin protein 22) is a growth-regulated membrane protein which is expressed by Schwann cells and is localized mainly in compact peripheral nervous system myelin. The gene which encodes PMP22 maps to human chromosome 17p11.2.