PRODUCT CODE: ET1704-99

Recombinant PLGF Monoclonal Antibody (ET1704-99)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of PLGF on SH-SY5Y cell lysate using anti-PLGF antibody at 1/1,000 dilution.
  • Western blot analysis of PLGF on SH-SY5Y cell lysate using anti-PLGF antibody at 1/1,000 dilution.
  • ICC staining PLGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PLGF in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PLGF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PLGF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PLGF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse orchis tissue using anti-PLGF antibody. Counter stained with hematoxylin.
Western blot analysis of PLGF on SH-SY5Y cell lysate using anti-PLGF antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PLGF Monoclonal Antibody (ET1704-99)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SH-SY5Y cell lysate, HepG2, MCF-7, rat kidney tissue, human colon cancer tissue, mouse orchis tissue, human liver cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA63-15

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

PLGF

SYNONYMS

D12S1900 antibody; Pgf antibody; PGFL antibody; PIGF antibody; Placenta growth factor antibody; Placental growth factor antibody; Placental growth factor, vascular endothelial growth factor related protein antibody; PlGF 2 antibody; PlGF antibody; PLGF_HUMAN antibody; PlGF2 antibody; SHGC 10760 antibody

SEQUENCE SIMILARITIES

Belongs to the PDGF/VEGF growth factor family.

TISSUE SPECIFICITY

While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

he onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factor (FGF), platelet derived growth factor (PDGF), vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF). Like VEGF, several PlGF variants have been shown to arise from alternative mRNA splicings. Evidence has suggested VEGF to be an obligatory component in PlGF signaling. While VEGF homodimers and VEGF/PlGF heterodimers function as potent mediators of mitogenic and chemotactic responses in endothelial cells, PlGF homodimers are effectual only at extremely high concentrations. Indeed, many of the physiological effects attributed to VEGF may actually be a result of VEGF/PlGF. VEGF and PlGF share a common receptor, Flt-1, and may also activate Flk-1/KDR.