PRODUCT CODE: ET1706-17

Recombinant Plexin A1 Monoclonal Antibody (ET1706-17)

  • Recombinant

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

ICC staining Plexin A1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Plexin A1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of LOVO cells with Plexin A1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
ICC staining Plexin A1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Plexin A1 Monoclonal Antibody (ET1706-17)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

LOVO.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU50-37

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

211 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

Plexin-A1

GENE NAME

PLXNA1

SYNONYMS

PLXNA1, NOV, PLXN1

SEQUENCE SIMILARITIES

Belongs to the plexin family.

TISSUE SPECIFICITY

Detected in fetal brain, lung, liver and kidney.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type I membrane protein.

FUNCTION

Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm (By similarity).