PRODUCT CODE: ET1607-80

Recombinant Plectin Monoclonal Antibody (ET1607-80)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Plectin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Plectin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Plectin antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Plectin antibody. Counter stained with hematoxylin.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Plectin Monoclonal Antibody (ET1607-80)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat skeletal muscle tissue, mouse skeletal muscle tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY29-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

531 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Plectin

SYNONYMS

EBS1 antibody; EBSO antibody; HD1 antibody; Hemidesmosomal protein 1 antibody; PCN antibody; pleC antibody; PLEC_HUMAN antibody; PLEC1 antibody; PLEC1b antibody; Plectin 1 antibody; Plectin 1 intermediate filament binding protein 500kDa antibody; Plectin 6 antibody; Plectin antibody; Plectin-1 antibody; PLTN antibody

SEQUENCE SIMILARITIES

Belongs to the plakin or cytolinker family.

TISSUE SPECIFICITY

Widely expressed with highest levels in muscle, heart, placenta and spinal cord.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.

SUBCELLULAR LOCATION

Cytoplasm, Cell junction.

FUNCTION

Plectin is an abundant cytoskeletal protein that is involved in cytoplasm stabilization. Plectin has been shown to crosslink intermediate filaments to microtubules and microfilaments, and to anchor intermediate filaments to the plasma and nuclear membranes. Plectin binds both Lamin B and vimentin, and this binding is regulated by a variety of protein kinases. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions. Plectin is also a substrate for p34cdc2 kinase. Several alternative splice isoforms of plectin are known to exist. Mutations in human plectin are known to cause epidermolysis bullosa simplex with muscular dystrophy (EBS-MD).