PRODUCT CODE: ET1705-21

Recombinant Placental alkaline phosphatase Monoclonal Antibody (ET1705-21)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of PLAP on human placenta tissue (1) and Hela cell (2) lysate using anti-PLAP antibody at 1/1,000 dilution.
  • Western blot analysis of PLAP on human placenta tissue (1) and Hela cell (2) lysate using anti-PLAP antibody at 1/1,000 dilution.
  • ICC staining PLAP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PLAP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PLAP antibody. Counter stained with hematoxylin.
Western blot analysis of PLAP on human placenta tissue (1) and Hela cell (2) lysate using anti-PLAP antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Placental alkaline phosphatase Monoclonal Antibody (ET1705-21)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human placenta tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM22-53

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Alkaline phosphatase, placental type

GENE NAME

ALPP

SYNONYMS

PLAP-1, ALPP, PLAP

SEQUENCE SIMILARITIES

Belongs to the alkaline phosphatase family.

TISSUE SPECIFICITY

Detected in placenta (at protein level).

SUBCELLULAR LOCATION

Cell membrane; Lipid-anchor, GPI-anchor.