PRODUCT CODE: ET1705-30

Recombinant PKC beta 1 Monoclonal Antibody (ET1705-30)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PKC beta 1 on L929 cell using anti-PKC beta 1 antibody at 1/1,000 dilution.
  • Western blot analysis of PKC beta 1 on L929 cell using anti-PKC beta 1 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC beta 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC beta 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PKC beta 1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with PKC beta 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of PKC beta 1 on L929 cell using anti-PKC beta 1 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PKC beta 1 Monoclonal Antibody (ET1705-30)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

L929, human tonsil tissue, human spleen tissue, mouse kidney tissue, K562.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM43-26

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

77 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PKC beta 1

SYNONYMS

BTN6 antibody; BTNL11 antibody; MGC26137 antibody; MOG alpha 5 antibody; MOG alpha 6 antibody; MOG AluA antibody; MOG AluB antibody; MOG antibody; MOG Ig AluB antibody; MOG_HUMAN antibody; MOGIG2 antibody; Myelin oligodendrocyte glycoprotein antibody; Myelin-oligodendrocyte glycoprotein antibody; NRCLP7 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade (By similarity).

SUBCELLULAR LOCATION

Cytoplasm. Nucleus. Membrane.

FUNCTION

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoformsandν. Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.