Positive control:
Lane 1: K562
Lane 2: 293
Applications
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WB
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ICC
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IF
-
IHC-P
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IP
-
FC
REACTIVITY
-
Human
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Mouse
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Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Recombinant PKC alpha Monoclonal Antibody (ET1608-15)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
K562, MCF-7, Hela, CRC, A549, PC12, mouse brain tissue, mouse small intestine tissue, mouse heart tissue, human lung tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU31-08
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
75 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:50-1:200
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IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
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IP
-
1:10-1:50
TARGET
UNIPROT #
PROTEIN NAME
PKC alpha
SYNONYMS
AAG6 antibody; Aging associated gene 6 antibody; aPKC antibody; KPCA_HUMAN antibody; PKC alpha antibody; PKC-A antibody; PKC-alpha antibody; PKCA antibody; PRKACA antibody; PRKCA antibody; Protein Kinase C alpha antibody; Protein kinase C alpha type antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Cell membrane, Mitochondrion membrane.
FUNCTION
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms. Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC and é are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.
CITATIONS
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Liu, Suzi et al.
Sinomenine protects against E.coli-induced acute lung injury in mice through Nrf2-NF-�B pathway. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2018]
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WB
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IHC
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mouse
-
Citation
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