PRODUCT CODE: ET1608-15

Recombinant PKC alpha Monoclonal Antibody (ET1608-15)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PKC alpha on different lysates using anti-PKC alpha antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: K562 <br />
  Lane 2: 293
  • Western blot analysis of PKC alpha on different lysates using anti-PKC alpha antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: K562 <br />
  Lane 2: 293
  • ICC staining PKC alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PKC alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PKC alpha in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PKC alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PKC alpha in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-PKC alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PKC alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PKC alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PKC alpha antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with PKC alpha antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of PKC alpha on different lysates using anti-PKC alpha antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: 293

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PKC alpha Monoclonal Antibody (ET1608-15)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, MCF-7, Hela, CRC, A549, PC12, mouse brain tissue, mouse small intestine tissue, mouse heart tissue, human lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU31-08

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

75 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

PKC alpha

SYNONYMS

AAG6 antibody; Aging associated gene 6 antibody; aPKC antibody; KPCA_HUMAN antibody; PKC alpha antibody; PKC-A antibody; PKC-alpha antibody; PKCA antibody; PRKACA antibody; PRKCA antibody; Protein Kinase C alpha antibody; Protein kinase C alpha type antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Cell membrane, Mitochondrion membrane.

FUNCTION

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms. Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC and é are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

CITATIONS

  • Liu, Suzi et al.

    Sinomenine protects against E.coli-induced acute lung injury in mice through Nrf2-NF-�B pathway. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2018]