PRODUCT CODE: ET1610-36

Recombinant PI3-kinase p110 subunit beta Monoclonal Antibody (ET1610-36)

  • Recombinant

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

Western blot analysis of PI3-kinase p110 subunit beta on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-36, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of PI3-kinase p110 subunit beta on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-36, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of PI3-kinase p110 subunit beta was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-36, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of PI3-kinase p110 subunit beta on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-36, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PI3-kinase p110 subunit beta Monoclonal Antibody (ET1610-36)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysates, human liver carcinoma tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC0580

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

123 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PI3-kinase p110 subunit beta

SYNONYMS

5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody; 5-bisphosphate 3-kinase catalytic subunit beta isoform antibody; DKFZp779K1237 antibody; MGC133043 antibody; OTTHUMP00000216901 antibody; OTTHUMP00000216904 antibody; p110 BETA antibody; p110Beta antibody; Phosphatidylinositol 3 kinase catalytic beta polypeptide antibody; Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta antibody; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform antibody; Phosphatidylinositol-4 antibody; Phosphoinositide 3 kinase catalytic beta polypeptide antibody; PI3 kinase p110 subunit beta antibody; PI3-kinase subunit beta antibody; PI3K antibody; PI3K beta antibody; PI3K-beta antibody; PI3Kbeta antibody; PI3KCB antibody; PIK3C1 antibody; Pik3cb antibody; PK3CB_HUMAN antibody; PtdIns 3 kinase p110 antibody; PtdIns-3-kinase subunit beta antibody; PtdIns-3-kinase subunit p110-beta antibody

SEQUENCE SIMILARITIES

Belongs to the PI3/PI4-kinase family.

TISSUE SPECIFICITY

Expressed ubiquitously.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-1070 down-regulates lipid kinase activity.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110 expression is restricted to white blood cells. It has been shown to bind p85α and β, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and β γ heterotrimeric G proteins.