Lane 1: MCF-7
Lane 2: Raji
Recombinant Rabbit monoclonal primary
Recombinant PI 3 Kinase p85 alpha Monoclonal Antibody (ET1608-70)
Raji, MCF-7, Hela, MCF-7, HepG2, NIH/3T3, mouse kidney tissue, mouse heart tissue, human liver cancer tissue, human kidney tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
PI 3 Kinase p85 alpha
GRB1 antibody; p85 alpha antibody; p85 antibody; P85A_HUMAN antibody; Phosphatidylinositol 3 kinase associated p 85 alpha antibody; Phosphatidylinositol 3 kinase regulatory 1 antibody; Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody; Phosphatidylinositol 3-kinase regulatory subunit alpha antibody; Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody; PI3 kinase p85 subunit alpha antibody; PI3-kinase regulatory subunit alpha antibody; PI3-kinase subunit p85-alpha antibody; PI3K antibody; PI3K regulatory subunit alpha antibody; Pik3r1 antibody; PtdIns 3 kinase p85 alpha antibody; PtdIns-3-kinase regulatory subunit alpha antibody; PtdIns-3-kinase regulatory subunit p85-alpha antibody
Belongs to the PI3K p85 subunit family.
Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.; Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.
Cytoplasm, membrane, nucleus.
Phosphatidylinositol 3-kinase (PI 3-kinase) phosphorylates the 3' OH position of the inositol ring of inositol lipids and is composed of p85 and p110 subunits. PI 3-kinase p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. PI 3-kinase p85α, also known as GRB1, phosphatidylinositol 3-kinase regulatory 1 or p85, is a 724 amino acid protein that exists as four alternatively spliced isoforms. Involved in insulin metabolism, defects in the PI 3-kinase p85α gene have been linked to insulin resistance. PI 3-kinase p85α is polyubiquitinated in T-cells by Cbl-b, and has multiple phosphorylated amino acid residues, including a phosphorylated tyrosine residue at position 467.
Cao, Pei et al.
The age-related changes and differences in energy metabolism and glutamate-glutamine recycling in the d-gal-induced and naturally occurring senescent astrocytes in vitro. | Experimental Gerontology