PRODUCT CODE: ET1607-74

Recombinant PI 3 Kinase Class 3 Monoclonal Antibody (ET1607-74)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PI 3 Kinase Class 3 on C2C12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of PI 3 Kinase Class 3 on C2C12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PI 3 Kinase Class 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-PI 3 Kinase Class 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PI 3 Kinase Class 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of PI 3 Kinase Class 3 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1607-74, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of PI 3 Kinase Class 3 on C2C12 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant PI 3 Kinase Class 3 Monoclonal Antibody (ET1607-74)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

C2C12 cell lysates, rat brain tissue, human stomach carcinoma tissue, mouse brain tissue, A549.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY0286

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

PI 3 Kinase Class 3

SYNONYMS

hVps34 antibody; MGC61518 antibody; Phosphatidylinositol 3 kinase catalytic subunit type 3 antibody; Phosphatidylinositol 3 kinase class 3 antibody; Phosphatidylinositol 3 kinase p100 subunit antibody; Phosphatidylinositol 3-kinase catalytic subunit type 3 antibody; Phosphatidylinositol 3-kinase p100 subunit antibody; Phosphoinositide 3 kinase class 3 antibody; Phosphoinositide-3-kinase class 3 antibody; PI3 kinase type 3 antibody; PI3-kinase type 3 antibody; PI3K type 3 antibody; Pik3c3 antibody; PK3C3_HUMAN antibody; PtdIns 3 kinase type 3 antibody; PtdIns-3-kinase type 3 antibody; Vps 34 antibody; Vps34 antibody

SEQUENCE SIMILARITIES

Belongs to the PI3/PI4-kinase family.

TISSUE SPECIFICITY

Ubiquitously expressed, with a highest expression in skeletal muscle.

SUBCELLULAR LOCATION

Midbody, Late endosome, Cytoplasmic vesicle.

FUNCTION

Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the 3' OH position of the inositol ring of inositol lipids. PI 3-kinase p100 (phosphoinositide-3-kinase p100 subunit), also known as hVps34 or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. PI 3-kinase p100 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. PI 3-kinase p100 exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. Unlike class I PI3Ks, whose activities are enhanced in the presence of magnesium, PI 3-kinase p100 activity is enhanced by manganese. Its activity can also be regulated by nutrients, suggesting an important role of PI-3 kinase p100 in the regulation of mTOR protein synthesis and autophagy.