PRODUCT CODE: ET1610-55

Recombinant Phospho SMC1(S957) Monoclonal Antibody (ET1610-55)

  • Recombinant

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

Western blot analysis of SMC1(phospho S957) on Hela cells lysates using anti- phospho -SMC1(S957) antibody at 1/1,000 dilution.
  • Western blot analysis of SMC1(phospho S957) on Hela cells lysates using anti- phospho -SMC1(S957) antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- phospho -SMC1(S957) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti- phospho -SMC1(S957) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti- phospho -SMC1(S957) antibody. Counter stained with hematoxylin.
Western blot analysis of SMC1(phospho S957) on Hela cells lysates using anti- phospho -SMC1(S957) antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Phospho SMC1(S957) Monoclonal Antibody (ET1610-55)

Immunogen

Recombinant protein

Host

Rabbit

Modification

Phospho

Modification Site

S957

Positive Control

Hela, human tonsil tissue, human lung cancer tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC65-03

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

160 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P:1:50-1:200

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Structural maintenance of chromosomes protein 1A

GENE NAME

SMC1A

SYNONYMS

SMC protein 1A, SMC-1-alpha, SMC-1A, SMC1A, SMC protein 1B, SMC-1-beta, SMC-1B, SMC1B

SEQUENCE SIMILARITIES

Belongs to the SMC family. SMC1 subfamily.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by the DCX(DCAF15) complex, leading to its degradation.; Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.

SUBCELLULAR LOCATION

Nucleus. Chromosome. Chromosome, centromere, kinetochore. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.

FUNCTION

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.