PRODUCT CODE: ET1611-22

Recombinant Phospho SHP2(Y542) Monoclonal Antibody (ET1611-22)

  • Recombinant

Applications

  • WB

  • ICC

  • IP

REACTIVITY

  • Human

  • Mouse

Western blot analysis of phospho -SHP2(Y542) on NIH/3T3 lysates using anti- phospho -SHP2(Y542) antibody at 1/1,000 dilution.
  • Western blot analysis of phospho -SHP2(Y542) on NIH/3T3 lysates using anti- phospho -SHP2(Y542) antibody at 1/1,000 dilution.
  • ICC staining phospho -SHP2(Y542) in B-6F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of phospho -SHP2(Y542) on NIH/3T3 lysates using anti- phospho -SHP2(Y542) antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IP

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Phospho SHP2(Y542) Monoclonal Antibody (ET1611-22)

Immunogen

Recombinant protein

Host

Rabbit

Modification

Phospho

Modification Site

Y542

Positive Control

NIH/3T3, B-6F1.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN61-01

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

68 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Tyrosine-protein phosphatase non-receptor type 11

GENE NAME

PTPN11

SYNONYMS

PTP-1D, PTP-2C, SHP-2, Shp2, PTPN11

SEQUENCE SIMILARITIES

Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.

TISSUE SPECIFICITY

Widely expressed, with highest levels in heart, brain, and skeletal muscle.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated on Tyr-542 and Tyr-580 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins. Phosphorylated upon activation of the receptor-type kinase FLT3. Phosphorylated upon activation of the receptor-type kinase PDGFRA (By similarity). Phosphorylated by activated PDGFRB.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. Positively regulates MAPK signal transduction pathway. Dephosphorylates GAB1, ARHGAP35 and EGFR. Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity. Dephosphorylates CDC73. Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity).