PRODUCT CODE: ET1608-53

Recombinant Phospho RSK1 p90 (T359+S363) Monoclonal Antibody (ET1608-53)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Phospho-RSK1 p90(T359+S363) on different lysates using anti-Phospho-RSK1 p90(T359+S363) antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: A431 <br />
  Lane 2: 293 <br />
  Lane 3: MCF-7
  • Western blot analysis of Phospho-RSK1 p90(T359+S363) on different lysates using anti-Phospho-RSK1 p90(T359+S363) antibody at 1/1,000 dilution.<br />
 Positive control: <br />
  Lane 1: A431 <br />
  Lane 2: 293 <br />
  Lane 3: MCF-7
  • ICC staining Phospho-RSK1 p90(T359+S363) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-RSK1 p90(T359+S363) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Phospho-RSK1 p90(T359+S363) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-RSK1 p90(T359+S363) antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-RSK1 p90(T359+S363) antibody. Counter stained with hematoxylin.
Western blot analysis of Phospho-RSK1 p90(T359+S363) on different lysates using anti-Phospho-RSK1 p90(T359+S363) antibody at 1/1,000 dilution.
Positive control:
Lane 1: A431
Lane 2: 293
Lane 3: MCF-7

Applications

  • WB

  • ICC

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Phospho RSK1 p90 (T359+S363) Monoclonal Antibody (ET1608-53)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding thr359 and ser363 of human rsk1 p90

Host

Rabbit

Modification

Phospho

Modification Site

T359+S363

Positive Control

A431, 293, HepG2, mouse liver tissue, human breast carcinoma tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU03-65

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

90 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Phospho-RSK1 p90(T359+S363)

SYNONYMS

90 kDa ribosomal protein S6 kinase 1 antibody; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1 antibody; dJ590P13.1 antibody; EC 2.7.11.1 antibody; HU 1 antibody; HU1 antibody; KS6A1_HUMAN antibody; MAP kinase activated protein kinase 1a antibody; MAP kinase-activated protein kinase 1a antibody; MAPK-activated protein kinase 1a antibody; MAPKAP kinase 1a antibody; MAPKAPK-1a antibody; MAPKAPK1A antibody; MGC79981 antibody; Mitogen-activated protein kinase-activated protein kinase 1A antibody; OTTHUMP00000004113 antibody; p90 RSK1 antibody; p90-RSK 1 antibody; p90rsk antibody; p90RSK1 antibody; p90S6K antibody; pp90RSK1 antibody; Ribosomal protein S6 kinase 90kD 1 antibody; Ribosomal protein S6 kinase 90kD polypeptide 1 antibody; Ribosomal protein S6 kinase 90kDa polypeptide 1 antibody; Ribosomal protein S6 kinase alpha 1 antibody; Ribosomal protein S6 kinase alpha-1 antibody; Ribosomal protein S6 kinase polypeptide 1 antibody; Ribosomal S6 kinase 1 antibody; RPS6K1 alpha antibody; rps6ka antibody; Rps6ka1 antibody; RSK 1 antibody; RSK 1 p90 antibody; RSK antibody; RSK-1 antibody; RSK1 antibody; S6K alpha 1 antibody; S6K-alpha-1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

POST-TRANSLATIONAL MODIFICATION

Activated by phosphorylation at Ser-221 by PDPK1. Autophosphorylated on Ser-380, as part of the activation process. May be phosphorylated at Thr-359 and Ser-363 by MAPK1/ERK2 and MAPK3/ERK1.; N-terminal myristoylation results in an activated kinase in the absence of added growth factors.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

The family of ribosomal S6 kinases (Rsks), designated Rsk-1 (or MAPKAP kinase-1), Rsk-2 and Rsk-3, are intracellular serine/threonine kinases that are important signaling intermediates in response to a broad range of ligand activated receptor tyrosine kinases. A unique feature common to the members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. An additional Rsk protein, Rsk-4, shows a high level of homology to the three previously isolated members of the human Rsk family. Rsk-4 is most abundantly expressed in brain and kidney and plays a role in normal neuronal development. The family of ribosomal S6 kinases includes p70 S6 kinase and p70 S6 kinase β, which are thought to have similar regulatory functions. MSK1 (also designated RLPK) is a novel Rsk-related protein, which, like the p90 Rsk family members, contains two non-identical complete kinase catalytic domains.