PRODUCT CODE: ET1611-18

Recombinant Phospho RPA32/RPA2 (Phospho T21) Monoclonal Antibody (ET1611-18)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of RPA32/RPA2 (phospho T21) on Hela cells lysates using anti-RPA32/RPA2 (phospho T21) antibody at 1/1,000 dilution.
  • Western blot analysis of RPA32/RPA2 (phospho T21) on Hela cells lysates using anti-RPA32/RPA2 (phospho T21) antibody at 1/1,000 dilution.
Western blot analysis of RPA32/RPA2 (phospho T21) on Hela cells lysates using anti-RPA32/RPA2 (phospho T21) antibody at 1/1,000 dilution.

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Recombinant Phospho RPA32/RPA2 (Phospho T21) Monoclonal Antibody (ET1611-18)

Immunogen

Recombinant protein

Host

Rabbit

Modification

Phospho

Modification Site

T21

Positive Control

Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN06-36

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

TARGET

UNIPROT #

PROTEIN NAME

Replication protein A 32 kDa subunit

GENE NAME

RPA2

SYNONYMS

RP-A p32, RF-A protein 2, RP-A p34, RPA2, REPA2, RPA32, RPA34

SEQUENCE SIMILARITIES

Belongs to the replication factor A protein 2 family.

POST-TRANSLATIONAL MODIFICATION

Differentially phosphorylated throughout the cell cycle, becoming phosphorylated at the G1-S transition and dephosphorylated in late mitosis. Mainly phosphorylated at Ser-23 and Ser-29, by cyclin A-CDK2 and cyclin B-CDK1, respectively during DNA replication and mitosis. Dephosphorylation may require the serine/threonine-protein phosphatase 4. Phosphorylation at Ser-23 and Ser-29 is a prerequisite for further phosphorylation. Becomes hyperphosphorylated on additional residues including Ser-4, Ser-8, Thr-21 and Ser-33 in response to DNA damage. Hyperphosphorylation is mediated by ATM, ATR and PRKDC. Primarily recruited to DNA repair nuclear foci as a hypophosphorylated form it undergoes subsequent hyperphosphorylation, catalyzed by ATR. Hyperphosphorylation is required for RAD51 recruitment to chromatin and efficient DNA repair. Phosphorylation at Thr-21 depends upon RFWD3 presence.; DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR. Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination.

SUBCELLULAR LOCATION

Nucleus. Nucleus, PML body. Note=Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner.

FUNCTION

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

CITATIONS

  • Bai, Yongtai et al.

    C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex. | Molecular Cell [2019]